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KMID : 0378019750180030301
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New Medical Journal
1975 Volume.18 No. 3 p.301 ~ p.310
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Toxicological Studies on Antitumor Agent Effects of L-Asparaginase on Gastric Secretion
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Abstract
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The effects of L-asparaginase on gastric secretion, liver, pancreas and blood pressure were ´studied with rats, rabbits and cats. Protein fractionations of L-asparaginases were performed by means of isoelectric focusing.
L-asparaginase (Bayer) had a tendency to depress gastric secretion in rats. Gastric pH was markedly elevated, while free acid concentration, output and total acid concentration were significantly decreased by L-asparaginase. J
In rabbits having ductal ligature, either pancreatic or common bile duct, L-asparaginase was administered intravenously in the dose ranges of 400 to 1, 600 u/kg. The liver and pancreas were examined 24 hours after treatment. Pancreatic ductal dilatation and interstitial inflammation were noted in the pancreatic duct ligated group, whereas liver infarction was found in the common bile duct ligated group.
In the group treated with L--asparaginase, in addition to ductal ligature, the changes were much like the group having ductal ligation alone.
Protein fractionations of L-asparaginases (Bayer and Merck, Sharp & Dohme) were separated by means of isoelectric focusing with Ampholine carrier ampholyte pH 3-10, and formed in a thin layer of polyacrylamide gel. Twenty one fractions with 5.25 as the main fraction´s isoelectric point (Ip) were identified with the preparation of Bayer (Crasnitine). Twenty four fractions with 5. 10 as the main fraction´s Ip were found using preparation of Merck, Sharp & Dohme (NSC 109229).
No changes in the blood pressure of cats were observed after the injection of L-asparaginase (Bayer) in the dose ranges of 100 to 2,000u/kg.
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